- By autor: Mohsin Amin
Single strand conformational polymorphism (SSCP) is a technique which is used to detect point mutation in genes. The purpose was to optimize the condition of SSCP for efficient and sensitive mutation detection in Exon 4, 5, 6, 7, 8, 9, 10, 11 and 12. All these Exon were amplified and run on agarose gel to check strong and specific amplification. These samples were then subjected for vertical elect...
Paperback: 68 pages
Publisher: LAP LAMBERT Academic Publishing (November 10, 2010)
Product Dimensions: 5.9 x 0.2 x 8.7 inches
Format: PDF ePub Text TXT fb2 book
Really great book, I would definitely recommend this book, if you don't have it, get it now. A great book for relaxing in your spare time. concrete engineering. book Sscp Conditions Optimization: Optimization Of Single Strand Conformational Polymorphism (Sscp)Conditions For Exon 4-12 Of Ldlr Gene Pdf
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for SSCP using different conditions of gel concentration, time, temperature and voltage to get maximum separation between single strands. Denaturation was carried out by mixing the samples with denaturant reagents. The length of nucleotide of Exon 4 was much longer than normal, so we amplified the exon 4 into two small parts named as 4a and 4b respectively after its bifurcation. The denaturation reagent consist of 50% 100mM NaOH and 50% Formamide loading buffer, 5?l-10?l sample was loaded in each case. In all the optimized Exons the denaturation was performed at 95°C for 7 minutes in PCR machine. This optimization will prove a valuable help in further study of the Exons of the same gene and other genes for Point Mutation Detection.